In vitro assessment of probiotic properties of <i>Pseudomonas entomophila</i><scp>COFCAU_PEP4</scp> isolated from <i>Labeo rohita</i> intestine

Assessment of beneficial properties by in vitro approaches forms the basis selection a potential probiotic. Moreover, bacterial strains autochthonous origin are considered more useful than from allochthonous sources. In this study, strain Pseudomonas entomophila COFCAU_PEP4, isolated intestine Indian major carp rohu (Labeo rohita), was evaluated for its probiotic using range tests, along with safety assessment. The showed antagonistic activity against 12 pathogenic indicator bacteria and tolerance to wide pH (2–9) bile salt concentrations (up 10%). also demonstrated significant adhesion properties, including mucosal adherence, auto-aggregation capacity, cell surface hydrophobicity. Besides being non-hemolytic, COFCAU_PEP4 able produce extracellular enzymes free radical scavenging potential. An experimental challenge study revealed as safe L. rohita. Taken together, assessments P. an source.